后面用逗号。
其写法类似于英文书信中的Dear ××× ，
正确写法是：Ladies and gentlemen，
即ladies首字母需要大写，gentleman小写，因为它并不是专有名词。
扩展资料：
参考范文：
Ladies and gentlemen,
First of all, please allow me, on behalf of all the members of my group, to express our heartfelt thanks to you for your kind invitation to visit your school. Thank you for giving us such a warm welcome.
Your school is one of the most famous high schools in the USA. We feel greatly honored to have such a chance to visit it today.
We are sure that our short stay here will be rich and colorful. We’d like to exchange experience in study with American students, and what’s more, we hope our American friends will give us some advice on how to learn English well.
Thanks again for your warm welcome.
参考资料：百度百科：英语演讲稿子
